In remembrance of Robert Rössle (* 1876   1956)





Robert Rössle has been endowed with
and has set an example to the virtue
of superior professorship (Doerr 1976):

Newton in science, Goethe in teaching!


His pioneering work dealt with introducing
objective standards and the quantification
of features that are relevant for the
description and diagnostic judgement
of organisms, organs, tissues
and even cells.
He was father of the scientific
investigation of processes involved
in inflammation and allergy.

The publication of his book was epochal:
 
Maß und Zahl in der Pathologie
(measure and number in pathology)
together with Frédérick Roulet, Berlin 1932.
























God did arrange everything as to number, measure and weight.
Institute of Chemistry in Leipzig at about 1864:
the front wall of the main lecture room (Quadbeck-Seeger 2007):


philosophy of this web presentation

This publication will be dealing with special topics of cytometric
interest in more detail, which might be helpful to understand,
why cytometry sometimes works but often fails.

Nevertheless, the old experience is valid still today:

Each failure has its reasons, each success its mystery.


historical remark on multi laser excitation in flow cytometry




multi

laser

flow

cytometry

in

the

beginning

The first cell sorter, equipped with a second laser, was established in 1975.
With  spatially  separated laser foci and solitary excitation (elapsed time)
two colour  fluorescence analysis could be performed using only one detec-
tor.


This instrument has been applied with various combinations of  fluorescent
dyes.  For the first time, the profit of Hoechst 33258 and DAPI - SR101 for
cell cycle  analyses of  fixed cells and the  Hoechst derivative  33662  to-
gether with PI for cycle phase determination of viable versus dead cells has
been demonstrated (Stoehr et al. 1976, 1977. 1980).
             - And this oldtimer is still working up to these days. -

This comes next

  • These web pages are currently under construction and are intended to provide some reflections on the mystery of quantitative fluorescent DNA staining and the key position of diffusion in selective binding as well as the accurate cytometric determination of the number of binding sites.